Activity of Ethanol Fraction Melinjo (Gnetum Gnemon L.) Seed on Colonic Cancer (Widr) Cells as Co-Chemotherapy Agent

Cancer deaths increase every year, including in Indonesia. The low selectivity of chemotherapy agents and the resistance cancer cells against is main cause treatment failure. It causes serious side effects sufferers. Beside that, plants produce secondary metabolites which are being investigated for anticancer activity that used as new clinical drugs. Therefore we need research uses co-chemotherapy agents. Melinjo seeds (Gnetum gnemon L.) contain gnetin C has potential to apoptosis WiDr colon cells. purpose this study was determine Ethanol Fraction Seed (EFMS) a agent cancer. sample maceration using 70% ethanol fractionated with ethanol. Phytochemical screening thin layer chromatography (TLC)-Densitometry, antioxidant test DPPH, while cytotoxic their combination 5-Fluorouracil 3-(4,-5-dimethylthiazo-2-yl)-2,5-diphenyltetrazolium bromide (MTT) Assay, also silico molecular docking between on EFMS IKK COX-2 proteins 5-FU. results showed contains based Rf value, weak IC50 1227 μg/mL, 681 μg/mL combined synergistic 5-Fluorouracil. Molecular strong binding affinity scores -12.2 kcal/mol -9.6 kcal/mol. result concludes inhibit development cells, especially cells.Keywords: Gnetum L., Colon Cancer, Cytotoxic, docking, WiDr.